建立小鼠肝脏体外复灌系统，并验证其有效性。

6只雄性C57小鼠获取肝脏后冷保存45 min内行体外复灌，复灌前获取同种属小鼠血液配制复灌液。小鼠复灌系统以多轴蠕动泵作为动力，实行门静脉单通道灌注，在灌注系统中加入输液器滴斗以缓冲压力和排气，2个小动物膜式氧合器串联以达到携氧要求，灌注系统总液体量约30 mL，复灌流量设定为2.5 mL·min^－1·g^－1。采用双托盘形式，满足小鼠肝脏正常体位。应用生物机能实验系统记录入肝压力等灌注参数，监测灌注液ALT、AST、肝组织病理及细胞凋亡等指标。

小鼠肝脏体外复灌过程中，门静脉压力低于8 mmHg，系统压力波动小于1 mmHg，灌注液pH值维持在7.40±0.05。复灌6 h结束时，灌注液ALT、AST和乳酸脱氢酶分别为(62±14)、(160±23)和(682±71) U/L，乳酸为(3.2±0.1) mmol/L。肝组织病理示肝小叶结构完整，实质细胞无明显坏死，病理学评分(1.1±0.7)分。原位缺口末端标记法检测肝细胞凋亡率为(0.57±0.22)%。

本研究国内首次构建了小鼠肝脏体外复灌系统，操作简单、运行稳定，能够较长时间维持肝脏正常生理功能。

To establish the reperfusion system in isolated mouse liver and test its effectiveness.

The isolated livers of 6 male C57 mice were reperfused in vitro with cold preservation time less than 45 minutes, and the blood obtained from allogenic mice was made up into perfusate before reperfusion. The power of reperfusion system came from peristaltic pump, and a dripping bucket was added for stress-buffering and venting. Two animal membrane oxygenation were in series with the reperfusion system to meet oxygen requirements. The total liquid of reperfusion system was about 30 mL, and the rate of reperfusion was set as 2.5 mL·min^-1·g^-1. Related parameters, such as liver perfusion pressure were recorded by biological functional experiment system, and indexes like ALT, AST of perfusate, histopathology of livers and cell apoptosis were also monitored.

During reperfusion in vitro, the portal vein pressure was lower than 8 mmHg, the system pressure fluctuation was lower than 1 mmHg, and the pH of the perfusion fluid was 7.40±0.05. At the end of reperfusion, the concentration of ALT, AST and lactate dehydrogenase of perfusion fluid were (62±14), (160±23) and (682±71) U/L, respectively, the concentration of lactic acid was (3.2±0.1) mmol/L. The pathology results of liver tissue showed that the structure of hepatic lobule was intact without obvious necrosis or apoptosis hepatocyte, and the pathological score was (1.1±0.7) scores. The apoptosis rate of hepatocyte was (0.57±0.22)%.

For the first time, the isolated reperfused mouse liver system was constructed. It is simple, stable, and could maintain normal physiological function of the liver for 6 hours.