Testing methods of non-human leukocyte antigen antibodies

doi:10.3877/cma.j.issn.1674-3903.2024.01.012

Abstract

Abstract:

Human leukocyte antigen (HLA) antibodies have been the focus in the prevention and treatment of antibody-mediated rejection (AMR) in organ transplantation. However, evidence from the clinic and basic studies has shown that non-HLA antibodies are associated with AMR or AMR like histologic lesions. There have been many methods to test non-HLA antibodies. Reliable and sensitive testing methods can help promote the application of non-HLA antibody detection in organ transplantation and improve clinical outcomes. This article review the research progress on testing methods of non-HLA antibodies.

Key words: Non-human leukocyte antigen antibodies, Organ transplantation, Antibody-mediated rejection, Antibody-mediated rejection like histologic lesions

Lihong Shang, Xiaotong Wu, Ning Li, Shaohua Shi, Zhihua Wang, Linru Zhu, Hua Zhou. Testing methods of non-human leukocyte antigen antibodies[J]. Chinese Journal of Transplantation(Electronic Edition), 2024, 18(01): 60-64.

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References 40

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性能	以HUVEC为底物的IIF法	以EPC为底物的流式细胞术	蛋白芯片技术	ELISA	单抗原Luminex微球试验
供者特异性	低	中等	低	低	低
抗原特异性	低	低	高	高	高
检测通量	高	高	高	低	中等
耗时	短	长	中等	少	少
成本(每样品)	低	中等	高	低	中等
筛选新型非HLA抗体	中等	中等	高	低	低

性能	以HUVEC为底物的IIF法	以EPC为底物的流式细胞术	蛋白芯片技术	ELISA	单抗原Luminex微球试验
供者特异性	低	中等	低	低	低
抗原特异性	低	低	高	高	高
检测通量	高	高	高	低	中等
耗时	短	长	中等	少	少
成本(每样品)	低	中等	高	低	中等
筛选新型非HLA抗体	中等	中等	高	低	低

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